SSR mining in coffee tree EST databases: potential use of EST–SSRs as markers for the Coffea genus
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چکیده
منابع مشابه
Exploiting EST databases for the mining and characterization of short sequence repeat (SSR) markers in Catharanthus roseus L.
Periwinkle (Catharanthus roseus L.) (Family: Apocyanaceae) is a ornamental plants with great medicinal properties. Although it is represented by seven species, little work has been carried out on its genetic characterization due to non-availability of reliable molecular markers. Simple sequence repeats (SSRs) have been widely applied as molecular markers in genetic studies. With the rapid incre...
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Coffee breeding and improvement efforts can be greatly facilitated by availability of a large repository of simple sequence repeats (SSRs) based microsatellite markers, which provides efficiency and high-resolution in genetic analyses. This study was aimed to improve SSR availability in coffee by developing new genic-/genomic-SSR markers using in-silico bioinformatics and streptavidin-biotin ba...
متن کاملIsolation and characterization of 22 EST-SSR markers for the genus Thujopsis (Cupressaceae)1
UNLABELLED • PREMISE OF THE STUDY Expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed from Thujopsis dolabrata var. hondae (Cupressaceae) using Illumina sequencing to investigate the genetic diversity and population structure of the genus Thujopsis. • METHODS AND RESULTS Twenty-two primer pairs were developed from ESTs of T. dolabrata var. hondae. The primers ampl...
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With the objective of developing new SSR markers for Coffea arabica, two enriched genomic libraries with probes (GT)15 and (AGG)10 were constructed. A total of 835 clones were sequenced and 756 presented good quality sequences. Redundant sequences were observed for 113 clones (14.94%). SSRs were found in 287 clones (38%). An estimated size of 417.5Kb of the C. arabica genome was sampled, with a...
متن کاملGenetic characterization of an elite coffee germplasm assessed by gSSR and EST-SSR markers.
Coffee is one of the main agrifood commodities traded worldwide. In 2009, coffee accounted for 6.1% of the value of Brazilian agricultural production, generating a revenue of US$6 billion. Despite the importance of coffee production in Brazil, it is supported by a narrow genetic base, with few accessions. Molecular differentiation and diversity of a coffee breeding program were assessed wi...
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ژورنال
عنوان ژورنال: Molecular Genetics and Genomics
سال: 2006
ISSN: 1617-4615,1617-4623
DOI: 10.1007/s00438-006-0153-5